SOC Medium for Bacterial Transformation

SOC medium is a nutritionally rich ready-to-use broth useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a bacteriological growth medium used for the transformation of competent cells (E. coli). This nutrient-rich broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation. More specifically SOC Medium can be used: in the propagation of DH-alpha (of E. coli strains) primarily in the recovery step of Escherichia coli competent cell transformations, and to maximize the transformation efficiency of competent cells. SOC medium formulation is SOB medium supplemented with 20mM glucose.   Recommendation Immediately before use, add 2M magnesium chloride to a final concentration of 20mM (10ml MgCl2/L).   Storage Conditions: 15°C to 30°C Shipping Condition: ambient temperature Autoclave Sterilized Max Shelf Life: 24 months from date of manufacture Harmonization Code: 3822.00.5090 UNSPSC: 41106200 ECCN: EAR99  

SOC Medium, For use in transformation, filtered Sigma-Aldrich

Bacterial Electrotransformation: An Interface Between Technology and Art

SOC Medium Biocompare

SOC Medium for Bacterial Transformation

Bacterial Transformation Protocols

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For the cultivation of recombinant strains of E.coli

SOC Medium Formato 500 g

Team:BGU Israel/Notebook1

Protocol for bacterial transformation – heat shock method – BBS OER Lab Manual