Schematic depiction of the single-step double recombination strategy.
![](https://www.researchgate.net/publication/259629323/figure/fig1/AS:213999313526784@1428032820506/Schematic-depiction-of-the-single-step-double-recombination-strategy-The-first.png)
![](https://www.frontiersin.org/files/Articles/886718/fcell-10-886718-HTML/image_m/fcell-10-886718-g002.jpg)
Frontiers V(D)J Recombination: Recent Insights in Formation of
![](https://www.researchgate.net/publication/44648522/figure/fig1/AS:365567665033216@1464169531225/Strategy-for-mutagenesis-of-the-apuA-gene-to-generate-the-knockout-mutant-apuA-spc.png)
Strategy for mutagenesis of the apuA gene to generate the knockout
Scheme of a two-step procedure to obtain gene-replacement
![](http://www.bx.psu.edu/~ross/workmg/RecombineDNACh8_files/image029.jpg)
III. RECOMBINATION (Lewin, Chpt. 33)
![](https://upload.wikimedia.org/wikipedia/commons/6/6d/Homologous_Recombination.jpg)
Genetic recombination - Wikipedia
![](https://media.addgene.org/data/easy-thumbnails/filer_public/cms/filer_public/6b/9a/6b9accc5-78ec-437e-b132-dd1c1d53715f/gateway_cloning.png__600x400_subsampling-2_upscale.png)
Addgene: Cloning
![](https://media.addgene.org/data/easy-thumbnails/filer_public/cms/filer_public/b4/c9/b4c9e9ca-5136-4c8c-96b5-a53d2e0dc0ad/restriction_cloning.png__900x353_crop_subsampling-2_upscale.png)
Addgene: Cloning
![](https://upload.wikimedia.org/wikipedia/commons/a/a2/DE_Mutations.png)
DNA shuffling - Wikipedia
![](https://www.researchgate.net/profile/Yukako-Hihara/publication/277519584/figure/fig6/AS:668516722151425@1536398215173/Effects-of-light-intensities-for-mixedliquid-culture-on-the-ratio-of-the-WL-to-WS_Q320.jpg)
Effects of light intensities for mixedliquid culture on the ratio of
![](https://www.mdpi.com/ijms/ijms-21-06461/article_deploy/html/images/ijms-21-06461-g001-550.jpg)
IJMS, Free Full-Text
![](https://www.nature.com/scitable/content/ne0000/ne0000/ne0000/ne0000/14459165/f2_huertas_nsmb.1710-f1.jpg)
Double-Strand DNA Breaks Learn Science at Scitable
![](https://www.investopedia.com/thmb/HM1n_tKDoyGfhqPLCRD3_VI3CA0=/1500x0/filters:no_upscale():max_bytes(150000):strip_icc()/dotdash_Final_MACD_and_Stochastic_A_Double_Cross_Strategy_Oct_2020-01-89c5f5e644054f9d91b00fc7fc6520b2.jpg)
MACD and Stochastic: A Double-Cross Strategy
![](https://www.researchgate.net/publication/24462583/figure/fig6/AS:668757580070919@1536455640724/Effects-of-light-intensities-for-mixedliquid-culture-on-the-ratio-of-the-WL-to-WS.png)
Effects of light intensities for mixedliquid culture on the ratio of
![](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs41598-022-09445-w/MediaObjects/41598_2022_9445_Fig1_HTML.png)
Efficient targeted transgenesis of large donor DNA into multiple
![](https://www.researchgate.net/publication/11012928/figure/fig1/AS:394612393955330@1471094334399/Models-for-the-repair-of-DSBs-A-In-the-DSBR-model-the-ends-are-processed-to-yield-3.png)
Models for the repair of DSBs. (A) In the DSBR model, the ends are